Gozo, Gharghur and Santa Venera were relegated from the Maltese Second Division 2009-10.
The winners of each section will win promotion and face each other in a championship play-off to decide the Maltese Third Division champions.
A total of twenty one teams divided into two sections will contest the league, including seventeen sides from the 2009–10 season, three relegated teams from the 2009–10 the Maltese Second Division and Marsaskala F.C., a new founded club.
Naxxar Lions had to beat Gudja Utd on penalties in the promotion play-off final to join them in the Second Division.
Mgarr Utd and Naxxar made their immediate return to the Second Division after being relegated at the end of the 2008–09 season.