These three teams were promoted from the 2009–10 Ukrainian Second League Two teams were relegated from the 2009–10 Ukrainian Premier League At the meeting of the Professional Football League of Ukraine, it was confirmed that FC Desna Chernihiv failed attestation for the season and hence would have their license withdrawn.
To allow an extra team to be promoted, the PFL determined that a playoff game between the 2nd placed teams from Druha Liha – Kremin Kremenchuk and Nyva Vinnytsia would determine the vacancy created.
At match one both second placed teams of the Second League groups played each other to determine the winner.
At match two the match was played between the 16th place team of the First League and the winner of another playoff game between the second placed clubs from each group of the Second League.
[36] At the end of the winter break Feniks-Illichivets Kalinino administration notified the PFL that their club was in liquidation and would withdraw from the league.