[1] The First Preliminary Round consists of teams from Druha Liha and Amateur Cup champions and has only five fixtures.

Tavriya Simferopol is the defending champion and as a member of the Premier League enter the competition at the round of 32.

[2] All matches consist of a single game and would include extra time and series of penalties if necessary to identify a winner.

The broadcast of the draw[3] was officially conducted for the first time by one of the sponsors of the Ukrainian Premier League (Footballua).

Clubs of a lower league when seeded with one from a higher would receive a home advantage.

A former Soviet player Vadym Yevtushenko was invited as a "special guest" to conduct the draw.

[5] ^(1) Match not played due to Ros Bila Tserkva's financial difficulties[6] ^(2) Qualify as Amateur Cup Champions of Ukraine 2009.

[7] ^(4) Initially the management of Feniks-Illichivets Kalinine informed their opponents that the club has removed itself from further competitions.

[8] However, four days later the club informed the PFL that their financial state had stabilized and that they would play.