The 2010 NCAA Division II women's basketball tournament was the 29th annual tournament hosted by the NCAA to determine the national champion of Division II women's collegiate basketball in the United States.

[1] Emporia State defeated Fort Lewis in the championship game, 65–53, to claim the Lady Hornets' first NCAA Division II national title.

The championship rounds were contested at the St. Joseph Civic Arena in St. Joseph, Missouri.

^ California, Pennsylvania Location: Hamer Hall Host: California University of Pennsylvania ~ Erie, Pennsylvania Location: Hammermill Center Host: Gannon University Location: Horne Arena Host: Lander University Location: First United Bank Center Host: West Texas A&M University Location: SDC Gymnasium Host: Michigan Technological University Location: Fitchburg State Recreation Center Host: Franklin Pierce College Location: Tucker Coliseum Host: Arkansas Tech University Location: Whalen Gymnasium Host: Fort Lewis College Location: Royal Brougham Pavilion Host: Seattle Pacific University Location: St. Joseph Civic Arena Host: Missouri Western State University