Bangkok and Rangsit JW were promoted to the 2011 Thai Division 1 League after winning the 2009 Regional League Division 2 championship pool.
Prachinburi were relegated from the 2010 Thai Division 1 League.
were effectively relegated from the division due to failure to meet Regional League standards.
Globlex, Maptaphut Rayong, Assumption College Thonburi, Royal Thai Fleet and Chamchuri United joined the newly expanded league setup.
Nonthaburi Provincial Stadium (Wat Bot Don Prom) ?