The 2015–16 USC Upstate Spartans women's basketball team represented the University of South Carolina Upstate in the 2015–16 NCAA Division I women's basketball season.

The Spartans, led by eleventh year head coach Tammy George, played their games at G. B. Hodge Center and are members of the Atlantic Sun Conference.

They advanced to the semifinals of the A-Sun women's tournament, where they lost to Jacksonville.

They were invited to the Women's Basketball Invitational, where they defeated Northern Kentucky in the first round, Western Illinois in the quarterfinals before losing to Weber State in the semifinals.

Non conference road games will typically be available on the opponents website.