The women's 400 metres hurdles event at the 2016 IAAF World U20 Championships was held at Zdzisław Krzyszkowiak Stadium on 20, 21 and 22 July.
Qualification: First 4 of each heat (Q) and the 4 fastest times (q) qualified for the semifinals.
[1] Qualification: First 2 of each heat (Q) and the 2 fastest times (q) qualified for the final.
[2] [3]