The 2016 Jackson State Tigers football team represented Jackson State University in the 2016 NCAA Division I FCS football season.

The Tigers were led by first-year head coach Tony Hughes.

They played their home games at Mississippi Veterans Memorial Stadium.

They were a member of the East Division of the Southwestern Athletic Conference.

They finished the season 3–8 overall and 3–6 in SWAC play to tie for third place in the East Division.