The 2016 Winnipeg National Bank Challenger was a professional tennis tournament played on outdoor hard courts.

It was the 1st edition, for men, and 4th edition, for women, of the tournament and part of the 2016 ATP Challenger Tour and the 2016 ITF Women's Circuit, offering totals of $75,000, for men, and $25,000, for women, in prize money.

It took place in Winnipeg, Manitoba, Canada between July 11 and July 17, 2016.

The following players received wildcards into the singles main draw: The following player entered the singles main draw with a protected ranking: The following players received entry from the qualifying draw: The following players received wildcards into the singles main draw: The following players received entry from the qualifying draw: