The Cardinals played their home games at Jim Patterson Stadium as a member of the Atlantic Coast Conference.

Following the conclusion of the regular season, the Cardinals were selected to host their fifth consecutive NCAA Regional.

With the Cardinals finishing the season as regular season champs of the Atlantic Division of the ACC, they would qualify for the 2016 NCAA Division I baseball tournament, and were selected as hosts of the Louisville regional and Louisville Super Regional, if they advanced to that round.

In the Super Regional, Louisville hosted UC Santa Barbara in a best-of-three matchup.

Louisville would lose the first two games to UC Santa Barbara, ending their playoff run.