The 2021 Big 12 Conference baseball tournament was held from May 25 through 30 at Chickasaw Bricktown Ballpark in Oklahoma City, Oklahoma.

The annual tournament determines the conference champion of the Division I Big 12 Conference for college baseball.

The winner of the tournament earned the league's automatic bid to the 2021 NCAA Division I baseball tournament.

There was a two-bracket double elimination tournament, leading to a winner-take-all championship game.

The 8th and 9th placed teams played in a play-in game to determine who will compete in the bracket.