The 2023–24 Youngstown State Penguins women's basketball team represented Youngstown State University during the 2023–24 NCAA Division I women's basketball season.

The Penguins, led by interim head coach John Nicolais, played their home games at the Beeghly Center in Youngstown, Ohio as members of the Horizon League.

On October 2, 2023, it was announced that head coach John Barnes would be taking a leave of absence to focus on "personal family matters," with associate head coach John Nicolais serving as acting head coach in Barnes' absence.

[1] On January 19, 2024, Barnes announced that he would be stepping down after ten years at the helm, with associate head coach John Nicolais serving as interim head coach for the remainder of the season.

[3] In the Horizon League tournament, they were defeated by Northern Kentucky in the quarterfinals.