The 2023 Open Rionegro was a professional tennis tournament played on clay courts.

It was the first edition of the tournament which was part of the 2023 ATP Challenger Tour.

It took place in Medellín, Colombia between 26 June and 1 July 2023.

The following players received wildcards into the singles main draw: The following players received entry into the singles main draw using protected rankings: The following players received entry from the qualifying draw: The following player received entry as a lucky loser: