[3] On February 28, 2023, having played the previous season wearing white shorts as part of the secondary kit, the club announced they were making the switch to black shorts to make players more comfortable when playing during their menstrual cycle.

Orlando were once again paired in the East region with Washington Spirit, North Carolina Courage and NJ/NY Gotham FC.

Reiss returned to Orlando having played with the Pride during the previous season before her contract expired.

[38] A further three trialists appeared during a preseason friendly against Kansas City Current on February 23: Jamaican international defender Konya Plummer returned having not played since her release by Orlando Pride in 2021, undrafted rookie Maliah Morris out of Clemson University, and Channing Foster who had been waived by Chicago Red Stars at the end of her rookie season in 2022.

[39] Rookie defender Brianna Martinez, who played five seasons at Notre Dame before being drafted by Racing Louisville FC in 2023, was also later added as a non-roster invitee during preseason.

[40] Kansas City Current draft pick Mykiaa Minniss also trained with the Pride during preseason.