[7] The winners of a play-in between the fifth-placed Six Nations team and the European champions were placed in WXV 2, while the losers were placed in WXV 3 alongside the winners of a play-off between the only two active Sudamérica Rugby women's national teams, Brazil and Colombia.

[12] In the opening match, Colombia scored thrice before half-time, including a double from Valentina Álvarez.

[13] Brazil mounted a comeback in the second half though, as the pressure of two yellow cards on Colombia's defence helped facilitate a controversial late try by Lohana Valente; evidence that she had grounded the ball was unclear.

[13][14] However, the attempt to convert it failed, and the match ended with Colombia leading the series by a single point.

[15][16] Two late penalty tries for Brazil were not enough to overcome the 54–42 aggregate score, and thus Colombia earned qualification to WXV 3.

[8][18][19] Italy played their first game at the Stadio Walter Beltrametti [it] and made a comfortable four-try defeat of Spain, and consequently earning qualification to WXV 2.

[18][20] Despite a notable offensive effort early in the second half, Spain failed to score a single point in the match, settling for a place in WXV 3.

[31] Samoa, Scotland and Italy played matches against Japan, South Africa and the United States.

The fourth and fifth team in the 2024 Women's Six Nations Championship will both automatically qualify for WXV 2 in 2024, with the sixth placed team facing the winner of the Rugby Europe Women's Championship for a place in WXV 2.