The 2024–25 James Madison Dukes women's basketball team represents the James Madison University during the 2024–25 NCAA Division I women's basketball season.

The Dukes, led by ninth-year head coach Sean O'Regan and play their home games at the Atlantic Union Bank Center in Harrisonburg, Virginia.

They are competing as members of the Sun Belt Conference.

[1] They were defeated by first-seeded Marshall in the championship game of the Sun Belt tournament in overtime.

[2] The Dukes then competed in the WBIT and lost to Stony Brook in the first round.