The 2020–21 James Madison Dukes women's basketball team represented James Madison University during the 2020–21 NCAA Division I women's basketball season.

The Dukes, led by fifth-year head coach Sean O'Regan, played their home games at the Atlantic Union Bank Center as members of the Colonial Athletic Association (CAA).

This was the first season the Dukes played their games at the new arena.

They received the number two seed in the CAA women's tournament and were eventually eliminated in the semifinal round of the tournament.

They were not invited to any additional post-season play.