The 2024–25 Stephen F. Austin Ladyjacks basketball team represents Stephen F. Austin State University during the 2024–25 NCAA Division I women's basketball season.

The Ladyjacks, who are led by second-year head coach Leonard Bishop, play their home games at the William R. Johnson Coliseum in Nacogdoches, Texas, as members of the Southland Conference (SLC).

The Southland Conference released its preseason poll on October 17, 2024.

Receiving 193 overall votes, the Ladyjacks were picked to finish fourth in the conference.

[1] Ashlyn Traylor was selected to the second-team Preseason All-Conference team.