The men's 400 metres hurdles at the 2024 European Athletics Championships took place at the Stadio Olimpico from 9 to 11 June.

[1] All times are local times (UTC+2) The next 12 fastest (q) advance to the semifinals.

[6] The first 2 in each heat (Q) and the next 2 fastest (q) advance to the final.

[7] *Athletes that received a bye into the semifinal