[3] On December 15, 2023, Major League Soccer announced that its teams would not participate in the 2024 edition of the U.S. Open Cup due to fixture congestion.
[4] The league intended to send reserve teams from MLS Next Pro instead, but the request was denied by the United States Soccer Federation on December 20.
[5] An agreement was tempered between the two sides in mid-February, whereby MLS will send some, but not all, of its teams to the Open Cup for 2024, with discussions ongoing for a long-term solution in 2025 and beyond.
[11] Metro areas with multiple teams: A total of 34 states were represented by clubs in the U.S. Open Cup this year.
Host team listed first Bold = winner The complete draw for the first round, including match dates and times, was announced on March 2, 2024.