The 2025 Punta del Este Open was a professional men's tennis tournament played on clay courts that took place between 20 and 26 January in Punta del Este, Uruguay.

[1] It was the fifth edition of the tournament and was part of the 2025 ATP Challenger Tour 75.

[2] The following players received wildcards into the singles main draw: The following players received entry into the singles main draw as alternates: The following players received entry from the qualifying draw: The following player received entry as a lucky loser: