The 2025 Rosario Challenger, known as the YPF Rosario Challenger, was a professional tennis tournament played on clay courts.

It was the first edition of the tournament, which was part of the 2025 ATP Challenger Tour.

It took place in Rosario, Argentina, between 3 and 9 February 2025.

[1] The following players received wildcards into the singles main draw: The following player received entry into the singles main draw as a special exempt: The following player received entry into the singles main draw as an alternate: The following players received entry from the qualifying draw: The following player received entry as a lucky loser: