The regiment was constituted on 1 February 1967 as the 214th Aviation Battalion, a Regular Army unit based at Fort Campbell, Kentucky.

It fought in the Vietnam War from 1967 to 1972, receiving the Meritorious Unit Commendation for its actions in 1967 and the Republic of Vietnam Gallantry Cross with Palm five times, in addition to the Republic of Vietnam Civil Action Honor Medal.

A battalion of the regiment fought in the Liberation of Kuwait campaign during the Gulf War.

[6] The 1st Battalion was inactivated at Los Alamitos on 15 September 1996, and became Company A, 214th Aviation while the rest of the 1st Battalion was disbanded on 16 September 1998, being simultaneously transferred to the Regular Army and activated in Germany.

[7] In March 2013, USAREUR announced that 1-214th Aviation was to be reduced at Clay Kaserne by 190 soldier spaces and at Landstuhl Regional Medical Center by 50 soldier spaces in 2016.