It was established in 2006 on the basis of a disbanded Separate Convoy Brigade, as the 48th regiment of the Internal Troops of Ukraine tasked mainly with phasing convicts into courts and prisons.

In April 2014, the 1st operational reserve battalion "Major General Serhiy Kulchytskyi" of National Guard of Ukraine, became a part of the brigade.

The brigade took part in the Siege of Sloviansk during which on 24 May 2014, separatists launched an armed attack on the checkpoint of the National Guard on the eastern outskirts of Sloviansk, near the intersection of the roads to Krasny Liman and Kramatorsk, the engagement lasted 2 hours involving the use of mortars and grenade launchers in addition to small arms during the engagement a soldier of the brigade (Zinchyk Stanislav Mykhailovych) was killed and some personnel were wounded as well.

The brigade performed combat support operations during the Battle of Mariupol during which on 5 September 2014 separatists attacked a column of Ukrainian forces arriving to Mariupol, near Boikivske, a soldier of the brigade (Oleksandr Ivanovych Zvinnyk) was killed in the attack[10] and another soldier (Eremenko Viktor Viktorovych) who was severely wounded died soon after on 11 September.

On 9 January 2015, while performing a combat mission near the Siverskyi Donets River, a battalion of the brigade was ambushed, in the ensuing two-hour battle, the attack was repulsed by backup from the other units of the National Guard and Armed Forces of Ukraine, during the battle a soldier of the brigade (Roman Ivanovych Lagno)[26] was killed and 14 more were wounded.