After fighting at First Manassas, the unit was assigned to General Pickett's, Garnett's, and Hunton's Brigade, Army of Northern Virginia.

[2] It was active in the campaigns of the army from Williamsburg to Gettysburg except when it served with Longstreet at Suffolk.

It fought at Cold Harbor, endured the battles and hardships of the Petersburg trenches, and was engaged in various conflicts around Appomattox.

[4][5] Private Marshall Sherman of the First Minnesota Infantry captured the regiment's battle flag at Gettysburg.

The field officers were Colonels Robert C. Allen, Robert T. Preston, and William Watts; Lieutenant Colonels Samuel B. Paul and William L. Wingfield; and Majors Michael P. Spesard and Nathaniel C. Wilson.