The 2nd Florida Cavalry Regiment was a Cavalry regiment from Florida that served in the Union Army between December 1863 and November 29, 1865, during the American Civil War.

During the summer of 1864, while the regiment was stationed at Fort Myers, many refugees in the town enlisted with the unit.

Expedition to Otter Creek, on Florida Railroad, October 30–31.

Fredrick Dyer mistook 2nd Florida Cavalry C.S.for this Unit.

2nd Florida Cavalry U.S. was not involved with the 17th Connecticut Volunteer Infantry in this Affair.]

Natural Bridge (in Leon County, Florida) March 6.