3,5-Lutidine is a heterocyclic organic compound with the formula (CH3)2C5H3N.

It is one of several dimethyl-substituted derivatives of pyridine, all of which are referred to as lutidines.

It is a colorless liquid with mildly basic properties and a pungent odor.

3,5-Lutidine is produced industrially by condensation of acrolein, ammonia, and formaldehyde:[1] The biodegradation of pyridines proceeds via multiple pathways.

[2] Although pyridine is an excellent source of carbon, nitrogen, and energy for certain microorganisms, methylation significantly retards degradation of the pyridine ring.