The sigma factors are important because they control how DNA promoter binding and RNA transcription start sites.

[4] The secondary structure consists of two irregular helical stem regions, making a large core loop which is called a central knot.

In many Pseudomonadota, 6S RNA may be processed from a transcript encoding homologs of the E. coli YgfA protein, which is a putative methenyltetrahydrofolate synthetase.

Strains with mutations in 6S RNA have a reduction of lifespan in contrast to the wild-type cells after more than 20 days of nonstop culture.

Gene expression studies revealed that 6S RNA is integrated in different global pathways e.g., it regulates various factors that influence transcription like Crp, FNR etc.

In a nutrient-deficient environment, 6S RNA control transcription leads to altered cell survival, possibly through redirecting resource consumption.

[13] Through SDS-PAGE analysis 6S RNA was identified present in E. coli and cover almost 25% of the total ribosomal number.

Numerous recent investigations have suggested that 6S RNA serves as a guardian, regulating the efficient utilisation of cellular resources under restricted conditions and stress.

So, the increase level of 6S RNA regulate alterations in gene expression are expected to aid adaptation to environmental challenges such as nutritional scarcity and high cell density.

6S RNA role in bacterial virulence has been identified that includes  L. pneumophila and Salmonella enterica serovar Typhimurium specifically where pathogenesis is linked to replication and stress resistance.