Company F was mustered in on September 13, 1862, at Louisville, Kentucky.

The regiment was attached to 17th Brigade, 3rd Division, Army of the Ohio, September 1862.

17th Brigade, 3rd Division, I Corps, Army of the Ohio, to November 1862.

2nd Brigade, 1st Division, Center, XIV Corps, Army of the Cumberland, to January 1863.

2nd Brigade, 1st Division, XIV Corps, Army of the Cumberland, to April 1863.

1st Brigade, 2nd Division, XIV Corps, to October 1863.

1st Brigade, 1st Division, XIV Corps, to June 1865.

The 88th Indiana Infantry mustered out of service on June 7, 1865.

Passage of the Cumberland Mountains and Tennessee River and Chickamauga Campaign August 16-September 22.

Davis Cross Roads or Dug Gap September 11.

Tunnel Hill, Buzzard's Roost Gap and Rocky Faced Ridge February 23–25.

Operations on line of Pumpkin Vine Creek and battles about Dallas, New Hope Church and Allatoona Hills May 25-June 5.

Operations about Marietta and against Kennesaw Mountain June 10-July 2.