The regiment fought against the forces of the Ottoman Empire, in Egypt, at Gallipoli, on the Sinai Peninsula, and in Palestine and Jordan.

During the inter-war years, the regiment was re-raised as a part-time unit based in South Australia, adopting the designation of the "Flinders Light Horse".

A unit of the all volunteer Australian Imperial Force, about two thirds of its recruits were from South Australia while the remainder came from Victoria.

In action one man of each section, was nominated as a horse holder reducing the regiment's rifle strength by a quarter.

[1] When the Australian infantry units were dispatched to Gallipoli, it was thought the terrain was unsuitable for mounted troops, and the light horse regiments remained in Egypt.

[6] The regiment was involved in the brigade's next attack at Hill 60 on 27 August, which resulted in the death of the replacement commanding officer Lieutenant Colonel Carew Reynell the next day.

[1] On the regiment's return to Egypt, together with the 3rd Light Horse Brigade, they were assigned to the newly raised ANZAC Mounted Division.

[9] The regiment was deemed surplus to requirements and, as part of a gradual demobilisation of the Australian Army, on 19 February 1943, it was disbanded without having seen operational service during the war.