During the war the regiment fought against the forces of the Ottoman Empire, in the Sinai and Palestine Campaign and was awarded fourteen battle honours.

During the inter-war years, the regiment was re-raised as a part-time unit based in the Northern Rivers region of New South Wales.

In action one man of each section, was nominated as a horse holder reducing the regiment's rifle strength by a quarter.

Over ten days the 5th Light Horse Brigade advanced 600 kilometres (370 mi) destroying road and rail links, and pursuing the retreating Ottoman Army into Syria.

The war cost the regiment sixteen killed and only three wounded, but for their service they were awarded fourteen battle honours, most of them inherited from the disbanded Imperial Camel Corps.