AG 489 (or agatoxin 489) is a component of the venom produced by Agelenopsis aperta,[1] a North American funnel web spider.

It inhibits the ligand gated ion channel TRPV1 through a pore blocking mechanism.

In result, the robust inhibitory activity was found in the venom.

Venom fractionation using reversed phase HPLC allowed the purification of two acylpolyamine toxins, AG489 and AG505.

Therefore, this was consistent with the scanned TM5-TM6 linker region[4] being the outer vestibule of the channels and further confirming that AG489 is a pore blocker.