The ANTH domain is a membrane binding domain that shows weak specificity for PtdIns(4,5)P2.

It was found in AP180 (homologous to CALM[1]) endocytotic accessory protein that has been implicated in the formation of clathrin-coated pits.

The domain is involved in phosphatidylinositol 4,5-bisphosphate binding and is a universal adaptor for nucleation of clathrin coats.

The PtdIns(4,5)P2 binding residues are spread over several helices at the tip of the structure.

An ANTH domain is also found in HIP1 and HIP1R, and the PtdIns(4,5)P2 binding sequence is conserved.