They compete in Serie D, the top tier of semi-professional Italian football after their promotion from the regional Eccellenza Tuscany league.

The first match in the major league was attended by Italian President Carlo Azeglio Ciampi, a Livorno citizen and team supporter in his childhood.

Donadoni was replaced by veteran coach Carlo Mazzone, who was only able to save a UEFA Cup place due to the expulsion of three teams from Europe in the Calciopoli scandal.

After a home loss to Rangers (2–3) and two 1–1 draws against Partizan in Belgrade (where goalkeeper Marco Amelia scored in the 87th minute) and Maccabi (in Livorno), the Tuscan side gained a 1–0 victory over Auxerre in the last game played in France, thus earning a spot in the Round of 32 of the competition.

His dismissal was, however, only delayed, as Arrigoni was eventually fired on 21 March 2007, and replaced by Fernando Orsi, who managed to keep the team away from the relegation battle.

For the 2007–08 campaign, Orsi was confirmed as head coach and a number of notable signings such as Francesco Tavano, Diego Tristan and Vikash Dhorasoo were finalised, but also the transfer of Lucarelli to Ukrainian club Shakhtar Donetsk.

On 28 April 2008, Camolese was fired as Orsi was re-appointed, but in the penultimate day of the season, the team could not avoid relegation, due to a 1–0 home defeat against Torino.

The club joined the Eccellenza Toscana for the 2021-22 season under the new denomination of Unione Sportiva Livorno 1915 and the ownership of former Prato chairman Paolo Toccafondi.

Former Lazio striker Paolo Di Canio once made a Roman salute to his own fans during a match against Livorno, when tensions were running high between the two clubs' ultra groups.

[8] Since 2005, a group of migrant Livorno supporters resident in northern Europe have styled themselves Partigiani Livornesi Scandinavia (transl.

A so-called "triangle of brotherhood" has developed between the most heavily supported left-wing fan clubs of Marseille, Livorno, and AEK Athens, namely between Commando Ultras 84, Brigate Autonome Livornesi 99, and Original 21.