The series, set in Frankfurt, features two main characters who solve crimes: a defense attorney and a private investigator.

In episode #149, Dr. Franck took a job as a law school teacher, and was replaced by Dr. Johannes Voss (played by Mathias Herrmann).

In episode #182, Dr. Voss was shot dead by an escaping criminal, and was in turn replaced by Dr. Markus Lessing (played by Paul Frielinghaus).

In late 2006 through early 2007, most episodes included scenes of Matula visiting a strip club, to interrogate the owner or one of the customers, with very brief footage of topless female dancers.

The show's structure is very similar to that of the American legal drama Perry Mason, though not many scenes in Ein Fall für zwei take place in a courtroom.

While the attorney he works with handles the juridistical part of the current case, Matula gets his hands dirty and tracks down the people he suspects of being involved in the crimes.

He has been arrested 12 times, and the entire plot of one episode centered on Matula being suspected of murder and having to escape the police into Austria.

Antoine Monot Jr. has since been playing attorney Benjamin "Benni" Hornberg alongside Wanja Mues who portrays investigator Leo Oswald.