Acadian Lines operated regular bus services between New Brunswick, Prince Edward Island and Nova Scotia as well as Rivière-du-Loup, and Quebec (connecting with Orléans Express).

[2] In 2011, Acadian Lines cancelled bus service on the route between Saint John, New Brunswick and Bangor, Maine due to low ticket sales.

[3][4] From 2 December 2011 until 16 May 2012 the company completely shut down its New Brunswick and Prince Edward Island operations after it locked out 59 drivers with Amalgamated Transit Union Local 1229 over a contract dispute.

Acadian Lines ceased operations in all three provinces on 30 November 2012, citing financial losses due to regulatory inflexibility for routes.

Additionally, the company has a subsidiary incorporated in the three provinces named Acadian Motor Coach Tours LP/Tours Autocars Acadien SEC.