Adherent culture

Adherent cell cultures with an excess of nutrient-containing growth medium will continue to grow until they cover the available surface area.

[7] The culture vessel can also be repeatedly tapped, which should be combined with either mechanical or enzymatic methods to facilitate cell detachment.

For adherent culture passaging, the spent media is first pipetted out of the flask containing cells as a waste product.

Heat is applied to the culture vessel for the incubation steps, causing protein denaturation and the gradual separation of the cells from the media.

Since their growth is limited to 2D, it is difficult to use adherent cultures to study in-vivo cell structure and function.