ADIX operates between Utica and Remsen over trackage of the Mohawk, Adirondack and Northern Railroad, part of the Genesee Valley Transportation Company.

The Remsen–Tupper Lake segment is owned by the State of New York and is designated as a multi-use corridor for rail traffic during the spring, summer, and fall seasons, and as a snowmobile trail during the winter months.

The section of track between Utica and Lyons Falls is used for freight traffic operated by the Mohawk, Adirondack and Northern Railroad (MA&N).

The line was built in 1892 by William Seward Webb, a Vanderbilt in-law,[2] as the Mohawk & Malone Railway and was purchased from him in 1893 by the New York Central and Hudson River Railroad.

[16] The first train ran over the entire 108-mile (174 km) Utica–Tupper Lake corridor in September 2022, and regular scheduled tourist excursion service began in late spring, 2023.