The lemur leaf frog participates in "prolonged breeding" that takes place continuously during the rainy seasons, primarily observed during spring or summer.

Tadpoles in earlier stages are smaller, slender, and less pigmented, and some even show an evident dark stripe between the eyes and nostrils.

With more than an 80% decrease in population in a period of 10 years it is now closely being monitored in Panama where it is still abundant in the lower elevations of central and eastern parts of the country.

Due to their marked drop in population these species of lemurs has been listed as critically endangered by the International Union for Conservation of Nature since 2004.

Related causes that may be linked to the disappearance of these frogs are chytridiomycosis (a disease affecting only amphibians contacting with zoospores of the Chytrid fungus) and general loss of habitat from deforestation.

Researchers are currently using non-invasive imaging technology to better understand how a specific species of tree frogs in Central America are proving resilience to this fungus.

[9] In 2004 a sample of peptides from nine different adult amphibians in Omar Torrijos National Park, Panama were taken to test for susceptibility to chytridiomycosis.

Of the 9 species tested the lemur leaf frog ranked third in immunologic resistance with a mean of 15% in inhibition of pathogen growth and peptide renewal.

The in-situ aspect of the project was carried out by the Costa Rican Amphibian Research Center, consisting of introducing tadpoles to artificial ponds every year, gradually increasing the wild population of the frogs inside of the reserve.

[14] Manchester University has also developed a global environmental education programme based on the species that extends to the countries of origin.

[15] The Atlanta Botanical Garden and El Valle, Panama have also bred the Panamanian form of the species in captivity and been highly successful.

Given that the population of the lemur leaf frog is on the decline in Central America, there are now clear efforts in place to help conserve the species.

In the necropsy of the lemur leaf frog that had died after 90 days, the Captive-Bred Juveniles (CBJ), (ones that included animals resulting from captive breeding) were compared with wild-caught Long-Term Residents (LTR).

The results showed that the cause of death for the lemur leaf frog at EVACC was through poor nutritional condition and osteodystrophy, with a very small number contracting lungworm infection and squamous metaplasia.

[16] A stumbling block to the conservation of lemur leaf frogs at El Valle in Panama has been its tendency to develop a vitamin A defiency when given insect-based diets in captivity.

At Manchester Museum and Bristol Zoo in the UK, and Nordens Ark in Sweden, they have successfully established a genetically diverse and healthy 'safety net' population of Costa Rican lemur leaf frogs.