It is native to the Guadalajara region of Jalisco state, and the Ceboruco volcano area of Nayarit state.

About 1–2 feet (0.3–0.6 m) in diameter, A. guadalajarana grows as a basal rosette of grey-green leaves with distinctive overlapment marks.

Agave guadalajarana is cultivated as an ornamental plant.

Easy to grow, it is best propagated by seed directly into the intended soil for the adult plant.

Multiple fertilizations is good during vegetation and the plant is best grown in a decently sized container.