The monastery, whose name means "Holy Trinity", was built in the 17th century by two brothers of the Venetian Zangaroli family on the site of a pre-existing church.

The main church is flanked by two smaller domed chapels, one of which is dedicated to the Life-Giving Spring (Zoodochos Pigi) and the other to Saint John the Theologian.

The facade of the church has double columns of Ionian and Corinthian style and bears an inscription in Greek, which is dated to 1631.

In the 19th century the monastery was established as an important theological school from 1833,[1] and the belfry is dated to 1864.

Important exhibits include a portable icon of St John the Theologian dated to around 1500, The Last Judgment, work of Emmanuel Skordiles from 17th century, St John the Precursor (1846), The Tree of Jesse (1853), The Hospitality of Abraham and The Descent into Hades (1855), The Story of Beauteaus Joseph (1858) and a manuscript on a parchment roll with the mass of St Basil.