[1] Like her four cousins, Edith, Alice, Margaret and Norah, Agnes Hamilton attended Miss Porter's School in Farmington, Connecticut.

[2] After the death of her father in 1895, together with her sister Jessie, attended the Pennsylvania Academy of the Fine Arts in Philadelphia from 1898 to 1900 studying under Cecilia Beaux.

Among her achievements: was a liaison between the First Presbyterian Church in Fort Wayne and Nebraska, a Mission School in a poor neighborhood;[2] was a leader in the Women's Club movement; was the leader of the Students' Art League; was the founder and first president of the Local YWCA;[1] opened the first library in Fort Wayne; was among the founders of the Bethany Presbyterian Church.

A statue to Edith, Alice and Agnes Hamilton is dedicated in Headwaters Park in downtown Fort Wayne.

[3] In 2005, Indiana University–Purdue University Fort Wayne formed the Society of Hamilton Sisters to honor the achievements, service contributions, and outstanding accomplishments of middle and high school girls.