Spanning 218.289 miles (351.302 km), the highway begins at the Mississippi state line at the terminus of Mississippi Highway 69 (MS 69) and connects the cities of Selma and Prattville before ending at SR 147 on the western side of Auburn.

SR 14 was one of the original routes in the Alabama's first statewide highway system in the 1920s.

The highway as built then started in Selma and traveled east along its current route to Auburn.

As was standard for highways of the era, SR 14 was unpaved for its full length.

In the 1960s, when the first segment of Interstate 85 (I-85) was constructed, bypassing Auburn and Opelika, SR 14 was extended from its eastern end at US 29 in Auburn along US 29 through Opelika to the northern end of that first Interstate Highway segment, at the current exit 64.