The Alabama and Tennessee River Railway (reporting mark ATN) is a shortline railway operating (via lease) over trackage formerly operated by CSX Transportation.

The parent company of the ATN is OmniTRAX, a major operator of American and Canadian short lines.

The route is a combination of the remnants of three former lines: the Seaboard Air Line's (SAL) Birmingham Subdivision from Birmingham to Wellington, the Louisville and Nashville Railroad (L&N) former Mineral Belt line from Birmingham to Gadsden, and the Nashville, Chattanooga and St. Louis Railway (NC&StL) line (ex-Tennessee and Coosa Railroad) from Guntersville to Gadsden, which once went further north to Huntsville using a car ferry over the Tennessee River.

Later, the SAL main line was abandoned from Wellington to Cedartown, Georgia.

[3] The line includes a short branch to Ivalee, Alabama which serves a Tyson Foods feed plant; this branch is a stub of the former L&N line to Birmingham.