It extends from the United States border at Wild Horse to Highway 55 in the hamlet of La Corey north of Bonnyville.

Highway 41 runs concurrent with Highway 1 for 16 kilometres (10 mi) before turning north at Dunmore, about 10 kilometres (6 mi) east of Medicine Hat.

Highway 41 travels in a northeasterly direction for about 65 kilometres (40 mi) before turning due north, parallel to the Saskatchewan border which is about 6.5 kilometres (4.0 mi) further east.

Highway 41 continues north for 20 kilometres (12 mi) and ends at Highway 55 at La Corey, about 40 kilometres (25 mi) west of Cold Lake.

41A is the designation of an alternate route off Highway 41 serving the City of Medicine Hat.