Peyrotte was born in Avignon, and was the son of a sculptor.
Early in his career he painted in the region of Carpentras parishes and congregations.
He participated with Joseph Duplessis to produce art decorations in the pharmacy of the Hôtel-Dieu of Carpentras.
His decorating work included apartments of the king and queen at Versailles (1738 and 1747), and the Board Room of the Palace of Fontainebleau with Charles-André van Loo, and the Château de Marly.
A number of his works were engraved and printed by Gabriel Huquier.