Actors who reprised their roles from the film include Mia Wasikowska (as Alice Kingsleigh), Crispin Glover (as Ilosovic Stayne,[6] the Knave of Hearts), Michael Sheen (as Nivens McTwisp the White Rabbit), Barbara Windsor (as Mallymkun the Dormouse), Stephen Fry (as Cheshire), and Leo Bill (as Hamish Ascot).

The Mad Hatter can help Alice alter her perception of Wonderland and take advantage of optical illusions to open up places in the world the player alone would not have noticed.

Meanwhile, the Cheshire Cat can use his ability to make himself and objects appear and disappear helping Alice through this strange world.

Players must choose wisely when using each of the characters' powers and combine the abilities to solve more complex puzzles.

For the DS, GameSpot criticized the combat and the occasional experience of not knowing what to do next, but praised the "visual direction", puzzles, characterization, humor, cleverness, and DSi features.