Her parents were the opera bassist Wenzel Michalesi (Czech: Václav Michalička; 1794-1836) and his wife Josefine (d 1845), a singer.
Her parents were members of the Prague Opera, leaving it in 1829 and embarking on art tours, eventually coming to the Staatstheater Mainz.
Later they were at the theatre in Brno, where Michalesi made her debut as Elvira in Mozart's Don Giovanni.
There she worked for several years in youthful soprano as well as contralto roles before moving to the Hamburg State Opera in 1846 after the death of her mother.
In 1850, she married the composer and Hofkapellmeister Karl August Krebs (1804–1880) and called herself Krebs-Michalesi from then on.