Amaseno (local dialect: Masé) is a comune (municipality) in the Province of Frosinone in the Italian region Lazio, located about 100 kilometres (62 mi) southeast of Rome and about 30 kilometres (19 mi) south of Frosinone, located in the Monti Lepini mountain area.

The Catholic Church situated at the village's center, St. Mary of Amaseno, houses a relic of St. Lawrence, the ancient Roman deacon martyred in the 3rd century.

The reliquary vial has been verified by scientists to contain true blood, along with a piece of skin and some fat and ash.

[citation needed] The cathedral was consecrated in 1165 and since then the village was popularly known as the "Valley of St. Lawrence", as the village is situated in an amphitheater-like valley.

The principal food product is the water buffalo mozzarella; other important products include olive oil and marzolina, a special cheese made by milk of goat.