It was originally built by the Reading Company as Wissahickon, until being renamed in 1869 after Mary Johnson Ambler, who helped direct the aftermath of the Great Train Wreck of 1856.

Its official address is at Butler Avenue and Main Street; however, the actual location is a block west on Butler Avenue and Short Race Street.

In FY 2017, Ambler station had a weekday average of 1,138 boardings and 881 alightings.

[5] In 2010, the station was moved south across Butler Pike into an entirely new ADA-accessible facility with long elevated platforms adjacent to the parking lots and a brand new ticket office, waiting room, and bathroom on the inbound side.

Media related to Ambler (SEPTA station) at Wikimedia Commons